Amphipolis was an ancient city located in Eastern Macedonia near Strymonas River, on the Thracian site once called Ennea Odoi (Nine Roads).

Amphipolis was founded by Athenians in 437 BC to exploit the Thracian mines. During the first phase of the Peloponnesian war (431 – 421 BC) the city of Amphipolis was handed over to the Spartans.

In the archaeological museum of Amphipolis, finds of prehistoric times to the Byzantine era are exhibited.